Lytic activity of LysH5 endolysin secreted by Lactococcus lactis using the secretion signal sequence of bacteriocin Lcn972.
نویسندگان
چکیده
Bacteriophage endolysins have an interesting potential as antimicrobials. The endolysin LysH5, encoded by Staphylococcus aureus phage vB_SauS-phi-IPLA88, was expressed and secreted in Lactococcus lactis using the signal peptide of bacteriocin lactococcin 972 and lactococcal constitutive and inducible promoters. Up to 80 U/mg of extracellular active endolysin was detected in culture supernatants, but most of the protein (up to 323 U/mg) remained in the cell extracts.
منابع مشابه
Title : Lytic activity of secreted LysH 5 endolysin by Lactococcus lactis using the 1 secretion signal
19 20 Bacteriophage endolysins have an interesting potential as antimicrobials. The endolysin 21 LysH5, encoded by Staphylococcus aureus phage vB_SauS-phi-IPLA88, was expressed 22 and secreted in Lactococcus lactis using the signal peptide of the bacteriocin lactococcin 23 972 and lactococcal constitutive and inducible promoters. Up to 80 U/mg of 24 extracelullar active endolysin was detected i...
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26 27 Phage-encoded endolysins are recently considered as new biocontrol tools to inhibit 28 pathogens in food. In this work, we have studied the ionic requirements for optimal lytic 29 activity of LysH5, the endolysin encoded by the staphylococcal bacteriophage phi30 SauS-IPLA88. LysH5 activity was inhibited by the presence of Mn and Zn and 31 enhanced by Ca, Mg and NaCl. When LysH5 was combin...
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 78 9 شماره
صفحات -
تاریخ انتشار 2012